Objective To investigate the application of best evidence for phase I cardiac rehabilitation in transcatheter aortic valve replacement（TAVR） patients into clinical practice and to evaluate its effectiveness. Methods The best evidence for phase I cardiac rehabilitation in patients with TAVR was summarized using an evidence-based care approach,and an evidence-based practice protocol was developed. The best evidence application strategy was constructed after a baseline review and analysis of barriers,and it was implemented in the cardiology department of a tertiary care hospital in Zhejiang Province from September 30 to December 31,2020. The indicators of patients before and after the application of evidence and the implementation rate of the reviewed entries were compared. Results The length of stay of patients after TAVR was shortened from 5（1,7） d at the baseline review to 3（1,6） d（P=0.016）. The percentage of cardiac function of New York Heart Association（NYHA） class I at 30 days postoperatively increased from 31.63% to 72.09%（P<0.001）,and the Katz index also improved（P=0.032）. After the application of best evidence,the rates of review indicators increased from 0~77.55% to 59.30%~100%,with a statistically significant difference（P<0.001）. Conclusion Best evidence application for phase I cardiac rehabilitation in patients undergoing TAVR can shorten patient length of stay,improve patient clinical outcomes,and standardize nurse behaviors.